Thermo Scientific Q33226 Qubit 4 Fluorometer

Product Description

The Invitrogen Qubit 4 Fluorometer (Catalog Number Q33226) is a compact, benchtop laboratory instrument engineered for the highly accurate quantitation of DNA, RNA, microRNA, and protein. Operating as a fluorescence-based system, it utilizes specialized Qubit assays to selectively bind to target molecules, ensuring precise measurements even in the presence of contaminants or degraded nucleic acids. Beyond standard quantitation, this model includes built-in functionality to evaluate RNA integrity and quality, as well as assess Ion Sphere Particle quality prior to sequencing runs. It operates entirely as a standalone device, requiring no external computer connection to manage data or perform calculations.

Key Features

• Target Selectivity: Employs target-specific fluorescence dyes to ensure accurate quantification of intact DNA, RNA, or protein, ignoring free nucleotides, short fragments, or degraded samples.
• Onboard Reagent Calculator: Includes an integrated digital tool that computes the exact volumes of master mix and buffer needed for a specified number of samples.
• RNA Quality Assessment: Features a built-in RNA Integrity and Quality (IQ) assay to quickly distinguish intact from degraded RNA.
• Custom Assays (MyQubit): Supports the upload of user-defined parameters via USB to configure custom fluorescence assays for specific target analytes.
• User Interface: Equipped with a 5.7-inch color touchscreen display for step-by-step experiment guidance and data manipulation.
• Rapid Processing: Capable of reading samples and returning concentration values in less than 5 seconds per sample.
• High Capacity Storage: Stores up to 1,000 individual sample records internally for easy retrieval and export.

Specifications

Optical System

• Light Sources: Blue LED (peak wavelength ~470 nm) and Red LED (peak wavelength ~635 nm)
• Excitation Filters: Blue (430–495 nm) and Red (600–645 nm)
• Emission Filters: Green (510–580 nm) and Red (665–720 nm)
• Detectors: Large-area photodiodes with measurement capabilities from 300 to 1,000 nm
• Dynamic Range: 5 orders of magnitude

Hardware and Sample Handling

• Sample Chamber: Accommodates one single 0.5-mL thin-wall clear PCR tube (Qubit Assay Tube)
• Minimum Sample Volume: Supports sample inputs from 1 µL to 20 µL
• Warm-up Time: Less than 35 seconds
• Data Ports: 1 x USB Type A (for flash drives) and 1 x USB Type mini-B (for direct computer connectivity)
• Memory Capacity: Includes a 4 GB USB flash drive for data management and transfer

Electrical and Environmental Settings

• Operating Power: 100–240 VAC, 1.0 A / 1.2 A
• Frequency: 50/60 Hz
• Electrical Input to Device: 12 VDC, 2.5 A via external AC adapter
• Installation Site: Indoor benchtop use only
• Operating Temperature: 10°C to 40°C
• Operating Humidity: 20% to 80% relative humidity, non-condensing

Weights and Dimensions

• Instrument Dimensions (W x L x H): 5.4 in. x 10.0 in. x 2.2 in. (13.6 cm x 25.0 cm x 5.5 cm)
• Instrument Weight: 26.2 oz (743 g)